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Crystallization and preliminary X-ray diffraction analysis of phospholipase A(1) isolated from hornet (Vespa basalis) venom

机译：Crystallization and preliminary X-ray diffraction analysis of phospholipase a(1) isolated from hornet (Vespa basalis) venom

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Phospholipase A(1) (PLA(1)) isolated from the black-bellied hornet (Vespa basalis) catalyzes the hydrolysis of emulsified phospholipids in addition to the potent haemolytic activity responsible for its lethal effect. In this study, the crystallization and preliminary crystallographic analysis of PLA1 from hornet venom with a molecular weight of 32 kDa are reported. PLA1 was crystallized at 277 K using PEG 4000 as precipitant and a 96.5% complete native data set was collected from a frozen crystal to 2.5 angstrom resolution at 100 K with an overall R(merge) of 6.8%. The crystal belongs to the triclinic space group P1, with unit-cell parameters a = 57.2, b = 70.2, c = 81.6 angstrom, alpha = 107.0, beta = 109.9, gamma = 100.9 degrees. In each asymmetric unit, three or four subunits of PLA(1) are present according to the calculation of the solvent content.

机译：从黑腹大黄蜂（Vespa basalis）分离出的磷脂酶A（1）（PLA（1））催化乳化磷脂的水解作用，此外还具有导致其致命作用的强溶血活性。在这项研究中，报道了从大黄蜂毒液中分子量为32 kDa的PLA1的结晶和初步晶体学分析。使用PEG 4000作为沉淀剂，将PLA1在277 K上结晶，并从冻结的晶体中以100 K分离至96的分辨率，获得了96.5％的完整天然数据，总R（合并）为6.8％。该晶体属于三斜晶空间群P1，单位晶胞参数a = 57.2，b = 70.2，c = 81.6埃，α= 107.0，β= 109.9，γ= 100.9度。在每个不对称单元中，根据溶剂含量的计算，存在三个或四个PLA（1）亚基。

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Chou, C.C.; Hou, M.H.; 侯明宏;
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Crystallization and preliminary X-ray diffraction analysis of phospholipase A(1) isolated from hornet (Vespa basalis) venom

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